Characterization and validation of an isotope-dilution LC–MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

Albarbarawi, O., Barton, A., Miller, D., McSharry, C., Chaudhuri, R. , Thomson, N. C., Palmer, C. N.A., Devereux, G. and Huang, J. T.-J. (2013) Characterization and validation of an isotope-dilution LC–MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum. Bioanalysis, 5(16), pp. 1991-2001. (doi: 10.4155/BIO.13.164)

Abstract

Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC–MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1–160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze–thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC–MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Thomson, Professor Neil and Chaudhuri, Dr Rekha and McSharry, Dr Charles
• Authors: Albarbarawi, O., Barton, A., Miller, D., McSharry, C., Chaudhuri, R., Thomson, N. C., Palmer, C. N.A., Devereux, G., and Huang, J. T.-J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Bioanalysis
• Publisher: Future Science
• ISSN: 1757-6180
• ISSN (Online): 1757-6199