T helper cell subsets specific for pseudomonas aeruginosa in healthy individuals and patients with cystic fibrosis

Bayes, H. K., Bicknell, S., MacGregor, G. and Evans, T. J. (2014) T helper cell subsets specific for pseudomonas aeruginosa in healthy individuals and patients with cystic fibrosis. PLoS ONE, 9(2), e90263. (doi:10.1371/journal.pone.0090263) (PMID:24587305) (PMCID:PMC3937364)

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Abstract

Background: We set out to determine the magnitude of antigen-specific memory T helper cell responses to Pseudomonas aeruginosa in healthy humans and patients with cystic fibrosis. Methods: Peripheral blood human memory CD4+ T cells were co-cultured with dendritic cells that had been infected with different strains of Pseudomonas aeruginosa. The T helper response was determined by measuring proliferation, immunoassay of cytokine output, and immunostaining of intracellular cytokines. Results: Healthy individuals and patients with cystic fibrosis had robust antigen-specific memory CD4+ T cell responses to Pseudomonas aeruginosa that not only contained a Th1 and Th17 component but also Th22 cells. In contrast to previous descriptions of human Th22 cells, these Pseudomonal-specific Th22 cells lacked the skin homing markers CCR4 or CCR10, although were CCR6+. Healthy individuals and patients with cystic fibrosis had similar levels of Th22 cells, but the patient group had significantly fewer Th17 cells in peripheral blood. Conclusions: Th22 cells specific to Pseudomonas aeruginosa are induced in both healthy individuals and patients with cystic fibrosis. Along with Th17 cells, they may play an important role in the pulmonary response to this microbe in patients with cystic fibrosis and other conditions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Evans, Professor Tom and Bicknell, Dr Stephen and MacGregor, Professor Gordon and Bayes, Dr Hannah
Authors: Bayes, H. K., Bicknell, S., MacGregor, G., and Evans, T. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS One 9(2):e90263
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
558681The Proinflammatory Th17 Response as a Therapeutic Target in Cystic Fibrosis Lung DiseaseTom EvansWellcome Trust (WELLCOME)094779/Z/10/ZIII - BACTERIOLOGY
558682The Proinflammatory Th17 Response as a Therapeutic Target in Cystic Fibrosis Lung DiseaseTom EvansWellcome Trust (WELLCOME)094779/Z/12AIII - BACTERIOLOGY