Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds

Alfotawi, R., Busuttil Naudi, K., Dalby, M. , Tanner, K.E. , McMahon, J. and Ayoub, A. (2013) Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds. Journal of Tissue Engineering, 4, p. 204173. (doi:10.1177/2041731413509645)

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Abstract

Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament™ Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ayoub, Professor Ashraf and Dalby, Professor Matthew and Busuttil Naudi, Dr Kurt and McMahon, Dr Jeremy and Tanner, Professor K
Authors: Alfotawi, R., Busuttil Naudi, K., Dalby, M., Tanner, K.E., McMahon, J., and Ayoub, A.
Subjects:R Medicine > RK Dentistry
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering
Publisher:Sage
ISSN:2041-7314
ISSN (Online):2041-7314
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Journal of Tissue Engineering 4:204173
Publisher Policy:Reproduced under a Creative Commons License

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