In response to pathogens, glial cells dynamically and differentially regulate Toll-like receptor gene expression

McKimmie, C. S. and Fazakerley, J. K. (2005) In response to pathogens, glial cells dynamically and differentially regulate Toll-like receptor gene expression. Journal of Neuroimmunology, 169(1-2), pp. 116-125. (doi: 10.1016/j.jneuroim.2005.08.006)

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Publisher's URL: http://dx.doi.org/10.1016/j.jneuroim.2005.08.006

Abstract

The mechanisms that mediate innate immune recognition of CNS infections are unknown. This study provides a comparison of Toll-like receptor (TLR) gene expression in resting and virus infected CNS cells. N2a neuroblastoma cells expressed TLR 3 but demonstrated no change in TLR gene expression in response to either LPS or virus infection. N9 microglia and differentiated primary astrocytes expressed most TLR genes. TLR 2 expression was highest in N9 microglia and TLR 7 in astrocytes. In both glial cell types, LPS stimulation upregulated pro-inflammatory cytokines, TLR 2 and TLR 3 gene expression but down-regulated other TLR genes. RNA virus infection substantially increased levels of type-I interferon (IFN) and TLR 3 transcripts and to a lesser extent TLR 9 transcripts. Microglia and astrocytes thus have the ability to discriminate between pathogens and elicit an appropriate response.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McKimmie, Dr Clive
Authors: McKimmie, C. S., and Fazakerley, J. K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Neuroimmunology
Publisher:Elsevier
ISSN:0165-5728
ISSN (Online):1872-8421

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