Innate immune response gene expression profiles of N9 microglia are pathogen-type specific

McKimmie, C. S., Roy, D., Forster, T. and Fazakerley, J. K. (2006) Innate immune response gene expression profiles of N9 microglia are pathogen-type specific. Journal of Neuroimmunology, 175(1-2), pp. 128-141. (doi: 10.1016/j.jneuroim.2006.03.012)

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Publisher's URL: http://dx.doi.org/10.1016/j.jneuroim.2006.03.012

Abstract

Glial cells, particularly microglia, are thought to play a pivotal role in initiating and guiding innate immune responses to CNS infections and in perpetuating inflammation and pathology in CNS diseases such as multiple sclerosis and Alzheimer's disease. We describe here the development and use of a new microarray designed to specifically profile transcript expression of innate immunity genes. Microarray analysis validated by quantitative PCR demonstrated an extensive range of pattern recognition receptor gene expression in resting N9 microglia, including Toll-like receptors, scavenger receptors and lectins. Stimulation with LPS or infection with virus modulated pattern recognition receptor, cytokine, chemokine and other innate immune transcripts in a distinct and stimulus-specific manner. This study demonstrates that a single glial cell phenotype has an innate capability to detect infection, determine its form and generate specific responses.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McKimmie, Dr Clive
Authors: McKimmie, C. S., Roy, D., Forster, T., and Fazakerley, J. K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Neuroimmunology
Publisher:Elsevier
ISSN:0165-5728
ISSN (Online):1872-8421

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