Using lymph node transplantation as an approach to image cellular interactions between the skin and draining lymph nodes during parasitic infections

Lawton, J. C., Benson, R. A., Garside, P. and Brewer, J. M. (2014) Using lymph node transplantation as an approach to image cellular interactions between the skin and draining lymph nodes during parasitic infections. Parasitology International, 63(1), pp. 165-170. (doi:10.1016/j.parint.2013.07.010)

Lawton, J. C., Benson, R. A., Garside, P. and Brewer, J. M. (2014) Using lymph node transplantation as an approach to image cellular interactions between the skin and draining lymph nodes during parasitic infections. Parasitology International, 63(1), pp. 165-170. (doi:10.1016/j.parint.2013.07.010)

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Abstract

The growing use of protozoan parasites expressing fluorescent reporter genes, together with advances in microscopy, is enabling visualisation of their behaviour and functions within the host from the very earliest stages of infection with previously unparalleled spatiotemporal resolution. These developments have begun to provide novel insights, which are informing our understanding of where host immune responses may be initiated, which cells are involved and the types of response that are elicited. Here we will review some of these recent observations that highlight the importance of cellular communication between the site of infection and the draining lymph node (dLN) in establishing infection and immunity. We also highlight a number of remaining challenges and unknowns that arise through our inability to follow and fate map the journey of a single cell between spatially separated tissue sites. In response to these challenges, we review a recently described experimental strategy that extends the spatial and temporal limits of previous imaging approaches, most significantly allowing longitudinal analysis of cellular migration between the skin and draining lymph nodes in vivo, without the requirement for invasive surgery.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Brewer, Professor James and Garside, Professor Paul and Lawton, Dr Jennifer and Benson, Dr Robert
Authors: Lawton, J. C., Benson, R. A., Garside, P., and Brewer, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Parasitology International
Publisher:Elsevier Ireland Ltd
ISSN:1383-5769
ISSN (Online):1873-0329
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Parasitology International 63(1):165-170
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/B/08/ZIII - PARASITOLOGY