Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse

Iwagaki, N., Garzillo, F., Polgár, E., Riddell, J.S. and Todd, A.J. (2013) Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse. Molecular Pain, 9(56), (doi:10.1186/1744-8069-9-56)

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Abstract

Background Inhibitory interneurons in the superficial dorsal horn play important roles in modulating sensory transmission, and these roles are thought to be performed by distinct functional populations. We have identified 4 non-overlapping classes among the inhibitory interneurons in the rat, defined by the presence of galanin, neuropeptide Y, neuronal nitric oxide synthase (nNOS) and parvalbumin. The somatostatin receptor sst2A is expressed by ~50% of the inhibitory interneurons in this region, and is particularly associated with nNOS- and galanin-expressing cells. The main aim of the present study was to test whether a genetically-defined population of inhibitory interneurons, those expressing green fluorescent protein (GFP) in the PrP-GFP mouse, belonged to one or more of the neurochemical classes identified in the rat.<p></p> Results The expression of sst2A and its relation to other neurochemical markers in the mouse was similar to that in the rat, except that a significant number of cells co-expressed nNOS and galanin. The PrP-GFP cells were entirely contained within the set of inhibitory interneurons that possessed sst2A receptors, and virtually all expressed nNOS and/or galanin. GFP was present in ~3-4% of neurons in the superficial dorsal horn, corresponding to ~16% of the inhibitory interneurons in this region. Consistent with their sst2A-immunoreactivity, all of the GFP cells were hyperpolarised by somatostatin, and this was prevented by administration of a selective sst2 receptor antagonist or a blocker of G-protein-coupled inwardly rectifying K+ channels.<p></p> Conclusions These findings support the view that neurochemistry provides a valuable way of classifying inhibitory interneurons in the superficial laminae. Together with previous evidence that the PrP-GFP cells form a relatively homogeneous population in terms of their physiological properties, they suggest that these neurons have specific roles in processing sensory information in the dorsal horn.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Iwagaki, Mr Noboru and Beresford-Polgar, Dr Erika and Todd, Professor Andrew and Riddell, Dr John
Authors: Iwagaki, N., Garzillo, F., Polgár, E., Riddell, J.S., and Todd, A.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Molecular Pain
Publisher:BioMed Central
ISSN:1744-8069
ISSN (Online):1744-8069
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Molecular Pain 9:56
Publisher Policy:Reproduced under a Creative Commons License

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