Nanotopographical effects on mesenchymal stem cell morphology and phenotype

Tsimbouri, P. , Gadegaard, N., Burgess, K., White, K., Reynolds, P., Herzyk, P. , Oreffo, R. and Dalby, M.J. (2014) Nanotopographical effects on mesenchymal stem cell morphology and phenotype. Journal of Cellular Biochemistry, 115(2), pp. 380-390. (doi:10.1002/jcb.24673)

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Abstract

There is a rapidly growing body of literature on the effects of topography and critically, nanotopography on cell adhesion, apoptosis and differentiation. Understanding the effects of nanotopography on cell adhesion and morphology and the consequences of cell shape changes in the nucleus, and consequently, gene expression offers new approaches to the elucidation and potential control of stem cell differentiation. In the current study we have used molecular approaches in combination with immunohistology and transcript analysis to understand the role of nanotopography on mesenchymal stem cell morphology and phenotype. Results demonstrate large changes in cell adhesion, nucleus and lamin morphologies in response to the different nanotopographies. Furthermore, these changes relate to alterations in packing of chromosome territories within the interphase nucleus. This, in turn, leads to changes in transcription factor activity and functional (phenotypical) signalling including cell metabolism. Nanotopography provides a useful, non-invasive tool for studying cellular mechanotransduction, gene and protein expression patterns, through effects on cell morphology. The different nanotopographies examined, result in different morphological changes in the cyto- and nucleo-skeleton. We propose that both indirect (biochemical) and direct (mechanical) signalling are important in these early stages of regulating stem cell fate as a consequence of altered metabolic changes and altered phenotype. The current studies provide new insight on cell–surface interactions and enhance our understanding of the modulation of stem cell differentiation with significant potential application in regenerative medicine.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew and Herzyk, Dr Pawel and Reynolds, Mr Paul and Burgess, Dr Karl
Authors: Tsimbouri, P., Gadegaard, N., Burgess, K., White, K., Reynolds, P., Herzyk, P., Oreffo, R., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Cellular Biochemistry
Publisher:Wiley
ISSN:0730-2312
ISSN (Online):1097-4644
Copyright Holders:Copyright © 2013 Wiley Periodicals, Inc.
First Published:First published in Journal of Cellular Biochemistry 115(2): 380-390
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
438161Stem Cell Differentiation & Genomic Processes in Response to Bioactive NanotopographyMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/G008868/1RI MOLECULAR CELL & SYSTEMS BIOLOGY