Shape-dependent optoelectronic cell lysis

Kremer, C., Witte, C., Neale, S. L. , Reboud, J. , Barrett, M. P. and Cooper, J. M. (2014) Shape-dependent optoelectronic cell lysis. Angewandte Chemie (International Edition), 53(3), pp. 842-846. (doi:10.1002/anie.201307751) (PMID:24402800) (PMCID:PMC4441254)

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Abstract

We show an electrical method to break open living cells amongst a population of different cell types, where cell selection is based upon their shape. We implement the technique on an optoelectronic platform, where light, focused onto a semiconductor surface from a video projector creates a reconfigurable pattern of electrodes. One can choose the area of cells to be lysed in real-time, from single cells to large areas, simply by redrawing the projected pattern. We show that the method, based on the “electrical shadow” that the cell casts, allows the detection of rare cell types in blood (including sleeping sickness parasites), and has the potential to enable single cell studies for advanced molecular diagnostics, as well as wider applications in analytical chemistry.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Barrett, Professor Michael and Reboud, Dr Julien and Witte, Dr Christian and Neale, Dr Steven
Authors: Kremer, C., Witte, C., Neale, S. L., Reboud, J., Barrett, M. P., and Cooper, J. M.
Subjects:Q Science > Q Science (General)
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Angewandte Chemie (International Edition)
Journal Abbr.:Angew Chem Int Ed Engl
Publisher:Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:1433-7851
ISSN (Online):1521-3773
Copyright Holders:Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
First Published:First published in Angewandte Chemie (International Edition) 53(3):842-846
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
387191Radical Solutions for Researching the Proteome (RASOR)Andrew PittBiotechnology and Biological Sciences Research Council (BBSRC)BB/C511572/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
387193Radical Solutions for Researching the Proteome (RASOR)Andrew PittBiotechnology and Biological Sciences Research Council (BBSRC)BB/C511572/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/B/08/ZIII - PARASITOLOGY