Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

Bernassau, A.L., Al-Rawhani, M., Beeley, J. and Cumming, D.R.S. (2013) Integrated ultrasonic particle positioning and low excitation light fluorescence imaging. Applied Physics Letters, 103(24), p. 244103. (doi: 10.1063/1.4846395)

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Abstract

A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent micro-particles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Al-Rawhani, Dr Mohammed and Cumming, Professor David and Bernassau, Dr Anne and Beeley, Dr James
Authors: Bernassau, A.L., Al-Rawhani, M., Beeley, J., and Cumming, D.R.S.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Applied Physics Letters
Publisher:American Institute of Physics
ISSN:0003-6951
ISSN (Online):1077-3118
Copyright Holders:Copyright © 2013 American Institute of Physics
First Published:First published in Applied Physics Letters 103(24):244103
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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