Evaluation of 24-color multifluor-fluorescence in-situ hybridisation (M-FISH) karyotyping by comparison with reverse chromosome painting of the human breast cancer cell line T-47D

Lu, Y.-J., Morris, J. , Edwards, P.A.W. and Shipley, J. (2000) Evaluation of 24-color multifluor-fluorescence in-situ hybridisation (M-FISH) karyotyping by comparison with reverse chromosome painting of the human breast cancer cell line T-47D. Chromosome Research, 8(2), pp. 127-132. (doi:10.1023/A:1009242502960)

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Abstract

Multifluor-fluorescence in-situ hybridization (M-FISH) chromosome paints for all the chromosomes in the human complement labeled with different combinations of fluorochromes is a recent technological development enabling assignment of chromosomal material to rearranged chromosomes. Little data is available on the accuracy and limitations of the approach to the analysis of complex karyotypes, which are characteristic of many malignant diseases. Here we compare M-FISH analysis of the breast-cancer-derived cell line T-47D with a previous analysis by reverse chromosome painting analysis of flow-sorted chromosomes from the same material. This demonstrated a high degree of concordance. It also illustrated the limitations of M-FISH analysis, including difficulties identifying small regions of chromosomal material and intrachromosomal rearrangements. Confirmation of selected aberrations using less-complex mixtures of painting probes and further definition of abnormalities using single copy markers may be required. The detailed karyotype description possible by M-FISH analysis contrasts with the definition in the original G-banding analysis. This and the level of concordance with reverse FISH painting supports the utility of the approach in the definition of complex karyotypes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Morris, Professor Joanna
Authors: Lu, Y.-J., Morris, J., Edwards, P.A.W., and Shipley, J.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Chromosome Research
ISSN:0967-3849
ISSN (Online):1573-6849

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