Singh, I., Wendeln, C., Clark, A.W. , Cooper, J.M. , Ravoo, B.J. and Burley, G.A. (2013) Sequence-selective detection of double-stranded dna sequences using pyrrole–imidazole polyamide microarrays. Journal of the American Chemical Society, 135(9), pp. 3449-3457. (doi: 10.1021/ja309677h)
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Abstract
We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole–imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide–alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burley, Dr Glenn and Cooper, Professor Jonathan and Clark, Professor Alasdair |
| Authors: | Singh, I., Wendeln, C., Clark, A.W., Cooper, J.M., Ravoo, B.J., and Burley, G.A. |
| College/School: | College of Science and Engineering > School of Chemistry College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Journal of the American Chemical Society |
| Publisher: | American Chemical Society |
| ISSN: | 0002-7863 |
| ISSN (Online): | 1520-5126 |
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