Sequence-selective detection of double-stranded dna sequences using pyrrole–imidazole polyamide microarrays

Singh, I., Wendeln, C., Clark, A.W. , Cooper, J.M. , Ravoo, B.J. and Burley, G.A. (2013) Sequence-selective detection of double-stranded dna sequences using pyrrole–imidazole polyamide microarrays. Journal of the American Chemical Society, 135(9), pp. 3449-3457. (doi:10.1021/ja309677h)

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Abstract

We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole–imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide–alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Clark, Dr Alasdair and Cooper, Professor Jonathan and Burley, Dr Glenn
Authors: Singh, I., Wendeln, C., Clark, A.W., Cooper, J.M., Ravoo, B.J., and Burley, G.A.
College/School:College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of the American Chemical Society
Publisher:American Chemical Society
ISSN:0002-7863
ISSN (Online):1520-5126

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