The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3)

Braceland, M., Bickerdike, R., Tinsley, J., Cockerill, D., Mcloughlin, M.F., Graham, D.A., Burchmore, R.J., Weir, W. , Wallace, C. and Eckersall, P.D. (2013) The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3). Journal of Proteomics, 94, pp. 423-436. (doi:10.1016/j.jprot.2013.10.016)

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Publisher's URL: http://dx.doi.org/10.1016/j.jprot.2013.10.016

Abstract

<br>Salmonid alphavirus is the aetological agent of pancreas disease (PD) in marine Atlantic salmon, Salmo salar, and rainbow trout, Oncorhynchus mykiss, with most outbreaks in Norway caused by SAV subtype 3 (SAV3). This atypical alphavirus is transmitted horizontally causing a significant economic impact on the aquaculture industry. This histopathological and proteomic study, using an established cohabitational experimental model, investigated the correlation between tissue damage during PD and a number of serum proteins associated with these pathologies in Atlantic salmon. The proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting. A number of humoral components of immunity which may act as biomarkers of the disease were also identified. For example, creatine kinase, enolase and malate dehydrogenase serum concentrations were shown to correlate with pathology during PD. In contrast, hemopexin, transferrin, and apolipoprotein, amongst others, altered during later stages of the disease and did not correlate with tissue pathologies. This approach has given new insight into not only PD but also fish disease as a whole, by characterisation of the protein response to infection, through pathological processes to tissue recovery.</br> <br>Biological significance: Salmonid alphavirus causes pancreas disease (PD) in Atlantic salmon, Salmo salar, and has a major economic impact on the aquaculture industry. A proteomic investigation of the change to the serum proteome during PD has been made with an established experimental model of the disease. Serum proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting with 72 protein spots being shown to alter significantly over the 12 week period of the infection. The concentrations of certain proteins in serum such as creatine kinase, enolase and malate dehydrogenase were shown to correlate with tissue pathology while other proteins such as hemopexin, transferrin, and apolipoprotein, altered in concentration during later stages of the disease and did not correlate with tissue pathologies. The protein response to infection may be used to monitor disease progression and enhance understanding of the pathology of PD.</br>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor Peter and Weir, Dr William and Braceland, Mr Mark
Authors: Braceland, M., Bickerdike, R., Tinsley, J., Cockerill, D., Mcloughlin, M.F., Graham, D.A., Burchmore, R.J., Weir, W., Wallace, C., and Eckersall, P.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Proteomics
Publisher:Elsevier
ISSN:1874-3919
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Journal of Proteomics 94: 423-436
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
530451Genotyping and Phenotyping Biomarkers of the Pathological Response to Pancreas Disease in the Atlantic Salmon.Peter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/I532837/1III - PARASITOLOGY