Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions

Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Köhler, J., and Freiberg, A. (2013) Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions. Journal of Physical Chemistry B, 117(40), pp. 12020-12029. (doi:10.1021/jp4073697)

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Abstract

The high sensitivity of optical spectra of pigment–protein complexes to temperature and pressure is well known. In the present study, we have demonstrated the significant influence of the environments commonly used in bulk and single-molecule spectroscopic studies at low temperatures on the LH2 photosynthetic antenna complex from Rhodopseudomonas acidophila. A transfer of this LH2 complex from a bulk-buffer solution into a spin-coated polymer film results in a 189 cm–1 blue shift of the B850 excitonic absorption band at 5 K. Within the molecular exciton model, the origin of this shift could be disentangled into three parts, namely to an increase of the local site energies, a contraction of the exciton band, and a decrease of the displacement energy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard and Southall, Mrs June
Authors: Kunz, R., Timpmann, K., Southall, J., Cogdell, R.J., Köhler, J.,, and Freiberg, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Physical Chemistry B
ISSN:1520-6106
ISSN (Online):1520-5207

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