Mapping an atlas of tissue-specific drosophila melanogaster metabolomes by high resolution mass spectrometry

Chintapalli, V.R., Al Bratty, M., Korzekwa, D., Watson, D.G. and Dow, J.A.T. (2013) Mapping an atlas of tissue-specific drosophila melanogaster metabolomes by high resolution mass spectrometry. PLoS ONE, 8(10), e78066. (doi:10.1371/journal.pone.0078066) (PMID:24205093) (PMCID:PMC3812166)

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Abstract

Metabolomics can provide exciting insights into organismal function, but most work on simple models has focussed on the whole organism metabolome, so missing the contributions of individual tissues. Comprehensive metabolite profiles for ten tissues from adult Drosophila melanogaster were obtained here by two chromatographic methods, a hydrophilic interaction (HILIC) method for polar metabolites and a lipid profiling method also based on HILIC, in combination with an Orbitrap Exactive instrument. Two hundred and forty two polar metabolites were putatively identified in the various tissues, and 251 lipids were observed in positive ion mode and 61 in negative ion mode. Although many metabolites were detected in all tissues, every tissue showed characteristically abundant metabolites which could be rationalised against specific tissue functions. For example, the cuticle contained high levels of glutathione, reflecting a role in oxidative defence; the alimentary canal (like vertebrate gut) had high levels of acylcarnitines for fatty acid metabolism, and the head contained high levels of ether lipids. The male accessory gland uniquely contained decarboxylated S-adenosylmethionine. These data thus both provide valuable insights into tissue function, and a reference baseline, compatible with the FlyAtlas.org transcriptomic resource, for further metabolomic analysis of this important model organism, for example in the modelling of human inborn errors of metabolism, aging or metabolic imbalances such as diabetes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dow, Professor Julian and Watson, Dr David and Chintapalli, Dr Venkateswara
Authors: Chintapalli, V.R., Al Bratty, M., Korzekwa, D., Watson, D.G., and Dow, J.A.T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in PLoS ONE 8(10):e78066
Publisher Policy:Reproduced under a Creative Commons License

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