Measuring chemotaxis using direct visualization microscope chambers

Muinonen-Martin, A.J., Knecht, D.A., Veltman, D.M., Thomason, P.A., Kalna, G. and Insall, R.H. (2013) Measuring chemotaxis using direct visualization microscope chambers. Methods in Molecular Biology, 1046, pp. 307-321. (doi:10.1007/978-1-62703-538-5_18)

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Abstract

Direct visualization chambers are considered the gold standard for measuring and analyzing chemotactic responses, because they allow detailed analysis of cellular behavior during the process of chemotaxis. We have previously described the Insall chamber, an improved chamber for measuring cancer cell chemotaxis. Here, we describe in detail how this system can be used to perform two key assays for both fast- and slow-moving mammalian and nonmammalian cell types. This allows for the detailed analysis of chemotactic responses in linear gradients at the levels of both overall cell behavior and subcellular dynamics.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Insall, Professor Robert and Muinonen-Martin, Dr Andrew and Thomason, Dr Peter and Kalna, Dr Gabriela and Knecht, Prof David
Authors: Muinonen-Martin, A.J., Knecht, D.A., Veltman, D.M., Thomason, P.A., Kalna, G., and Insall, R.H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Methods in Molecular Biology
ISSN:1064-3745

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