Baldini, F. , Gabrieli, P., South, A., Valim, C., Mancini, F. and Catteruccia, F. (2013) The interaction between a sexually transferred steroid hormone and a female protein regulates oogenesis in the malaria mosquito anopheles gambiae. PLoS Biology, 11(10), e1001695. (doi: 10.1371/journal.pbio.1001695)
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Abstract
Molecular interactions between male and female factors during mating profoundly affect the reproductive behavior and physiology of female insects. In natural populations of the malaria mosquito Anopheles gambiae, blood-fed females direct nutritional resources towards oogenesis only when inseminated. Here we show that the mating-dependent pathway of egg development in these mosquitoes is regulated by the interaction between the steroid hormone 20-hydroxy-ecdysone (20E) transferred by males during copulation and a female Mating-Induced Stimulator of Oogenesis (MISO) protein. RNAi silencing of MISO abolishes the increase in oogenesis caused by mating in blood-fed females, causes a delay in oocyte development, and impairs the function of male-transferred 20E. Co-immunoprecipitation experiments show that MISO and 20E interact in the female reproductive tract. Moreover MISO expression after mating is induced by 20E via the Ecdysone Receptor, demonstrating a close cooperation between the two factors. Male-transferred 20E therefore acts as a mating signal that females translate into an increased investment in egg development via a MISO-dependent pathway. The identification of this male–female reproductive interaction offers novel opportunities for the control of mosquito populations that transmit malaria.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Baldini, Dr Francesco |
Authors: | Baldini, F., Gabrieli, P., South, A., Valim, C., Mancini, F., and Catteruccia, F. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | PLoS Biology |
Publisher: | Public Library of Science |
ISSN: | 1544-9173 |
ISSN (Online): | 1545-7885 |
Copyright Holders: | Copyright © 2013 The Authors |
First Published: | First published in PLoS Biology 11(10):e1001695 |
Publisher Policy: | Reproduced under a Creative Commons License |
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