A facile quantitative assay for viral particle genesis reveals cooperativity in virion assembly and saturation of an antiviral protein

Yadav, S.S., Wilson, S.J. and Bieniasz, P.D. (2012) A facile quantitative assay for viral particle genesis reveals cooperativity in virion assembly and saturation of an antiviral protein. Virology, 429(2), pp. 155-162. (doi: 10.1016/j.virol.2012.04.008)

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Abstract

Conventional assays of viral particle assembly and release are time consuming and laborious. We have developed an enzymatic virus-like particle (VLP) genesis assay that rapid and quantitative and is also versatile and applicable to diverse viruses including HIV-1 and Ebola virus. Using this assay, which has a dynamic range of several orders of magnitude, we show that the efficiency of VLP assembly and release, i.e., the fraction of the expressed protein that is assembled into extracellular particles, is dependent on the absolute level of expression of either HIV-1 Gag or Ebola virus VP40. We also demonstrate that the activity of the antiviral factor tetherin is dependent on the level of HIV-1 Gag expression and the numbers of VLPs generated, and appears to become saturated as these parameters are increased.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Professor Sam
Authors: Yadav, S.S., Wilson, S.J., and Bieniasz, P.D.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Virology
Publisher:Elsevier
ISSN:1096-0341
ISSN (Online):0042-6822
Copyright Holders:Copyright © 2012 Elsevier Inc.
First Published:First published in Virology 429(2)155-162
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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