A multi-modal stereo microscope based on a spatial light modulator

Lee, M.P., Gibson, G.M. , Bowman, R., Bernet, S., Ritsch-Marte, M., Phillips, D. and Padgett, M.J. (2013) A multi-modal stereo microscope based on a spatial light modulator. Optics Express, 21(14), pp. 16541-16551. (doi: 10.1364/OE.21.016541)

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Abstract

Spatial Light Modulators (SLMs) can emulate the classic microscopy techniques, including differential interference (DIC) contrast and (spiral) phase contrast. Their programmability entails the benefit of flexibility or the option to multiplex images, for single-shot quantitative imaging or for simultaneous multi-plane imaging (depth-of-field multiplexing). We report the development of a microscope sharing many of the previously demonstrated capabilities, within a holographic implementation of a stereo microscope. Furthermore, we use the SLM to combine stereo microscopy with a refocusing filter and with a darkfield filter. The instrument is built around a custom inverted microscope and equipped with an SLM which gives various imaging modes laterally displaced on the same camera chip. In addition, there is a wide angle camera for visualisation of a larger region of the sample.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gibson, Dr Graham and Phillips, Dr David and Padgett, Professor Miles
Authors: Lee, M.P., Gibson, G.M., Bowman, R., Bernet, S., Ritsch-Marte, M., Phillips, D., and Padgett, M.J.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
Copyright Holders:Copyright © 2013 Optical Society of America
First Published:First published in Optics Express 21(4):16541-16551
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
549871Challenges in Orbital Angular MomentumMiles PadgettEngineering & Physical Sciences Research Council (EPSRC)EP/I012451/1P&A - PHYSICS & ASTRONOMY