Enlighten
Research publications by members of the University of Glasgow
home > services > Enlighten

Isotopic composition of inorganic carbon as an indicator of benzoate degradation by Pseudomonas putida: temperature, growth rate and pH effects

Barth, J.A.C., Kalin, R.M., Larkin, M.J., Hall, J.A., and Fitzgerald, U. (2000) Isotopic composition of inorganic carbon as an indicator of benzoate degradation by Pseudomonas putida: temperature, growth rate and pH effects. Rapid Communications in Mass Spectrometry, 14 (15). pp. 1316-1320. ISSN 0951-4198 (doi:10.1002/1097-0231(20000815)14:15<1316::AID-RCM933>3.0.CO;2-4)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1002/1097-0231(20000815)14:15<1316::AID-RCM933>3.0.CO;2-4

Abstract

Degradation experiments of benzoate by Pseudomonas putida resulted in enzymatic carbon isotope fractionations, However, isotopic temperature effects between experiments at 20 and 30 degrees C were minor. Averages of the last three values of the CO2 isotopic composition (delta(13)C(CO2(g))) were more negative than the initial benzoate delta(13)C value (-26.2 parts per thousand Vienna Pee Dee Belenite (VPDB)) by 3.8, 3.4 and 3.2 parts per thousand at 20, 25 and 30 degrees C, respectively. Although the maximum isotopic temperature difference found was only 0.6 parts per thousand, more extreme temperature variations may cause larger isotope effects. In order to understand the isotope effects on the total inorganic carbon (TIC), a better measure is to calculate the proportions of the inorganic carbon species (CO2(g), CO2(aq) and HCO3-) and to determine their cumulative delta(13)C(TIC). In all three experiments delta(13)C(TIC) was more positive than the initial isotopic composition of the benzoate at a pH of 7, This suggests an uptake of C-12 in the biomass in order to match the carbon balance of these closed system experiments.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):UNSPECIFIED
Authors: Barth, J.A.C., Kalin, R.M., Larkin, M.J., Hall, J.A., and Fitzgerald, U.
Subjects:Q Science > QD Chemistry
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Rapid Communications in Mass Spectrometry
ISSN:0951-4198

University Staff: Request a correction | Enlighten Editors: Update this record