Human and mouse macrophage-inducible C-type lectin (Mincle) bind Candida albicans

Bugarcic, A., Hitchens, K., Beckhouse, A.G., Wells, C.A., Ashman, R.B. and Blanchard, H. (2008) Human and mouse macrophage-inducible C-type lectin (Mincle) bind Candida albicans. Glycobiology, 18(9), pp. 679-685. (doi:10.1093/glycob/cwn046)

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Publisher's URL: http://dx.doi.org/10.1093/glycob/cwn046

Abstract

Candida albicans is a causative agent in mycoses of the skin, oral cavity, and gastrointestinal tract. Identification of receptors, and their respective ligands, that are engaged by immune cells when in contact with C. albicans is crucial for understanding inflammatory responses leading to invasive candidiasis. Mincle is a recently identified macrophage-expressed receptor that is important for host responses to C. albicans. The carbohydrate-recognition domain of human and mouse Mincle were expressed, purified under denaturing conditions, and successfully refolded. In addition to oligomers, there are isolatable monomeric and dimeric forms of the protein that occur under two different buffer solutions. The human and mouse homologues bound yeast extract, and the isolated dimeric and monomeric species also demonstrated the recognition of whole C. albicans yeast cells. The data are indicative of several functional states mediating the interaction of Mincle and yeast at the surface of the macrophage.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wells, Dr Christine
Authors: Bugarcic, A., Hitchens, K., Beckhouse, A.G., Wells, C.A., Ashman, R.B., and Blanchard, H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Glycobiology
ISSN:0959-6658
ISSN (Online):1460-2423

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