The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

Davidson, A.J., King, J.S. and Insall, R.H. (2013) The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum. BioTechniques, 55(1), pp. 39-41. (doi:10.2144/000114054)

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Abstract

The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development. In addition, Dictyostelium-specific antibodies for other potential control proteins are not commercially available. Here we demonstrate the use of labeled streptavidin to detect biotinylated mitochondrial 3-methylcrotonyl-CoA carboxylase α (MCCC1), providing a robust and convenient tool for quantitative normalization of Dictyostelium Western blots, as well as fluorescently labeling mitochondria for microscopy of fixed cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Insall, Professor Robert and King, Dr Jason and Davidson, Mr Andrew
Authors: Davidson, A.J., King, J.S., and Insall, R.H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:BioTechniques
ISSN:1940-9818
ISSN (Online):1940-9818

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