In Vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis

Zanivan, S. et al. (2013) In Vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis. Cell Reports, 3(2), pp. 552-566. (doi:10.1016/j.celrep.2013.01.003)

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Abstract

Cancer progresses through distinct stages, and mouse models recapitulating traits of this progression are frequently used to explore genetic, morphological, and pharmacological aspects of tumor development. To complement genomic investigations of this process, we here quantify phosphoproteomic changes in skin cancer development using the SILAC mouse technology coupled to high-resolution mass spectrometry. We distill protein expression signatures from our data that distinguish between skin cancer stages. A distinct phosphoproteome of the two stages of cancer progression is identified that correlates with perturbed cell growth and implicates cell adhesion as a major driver of malignancy. Importantly, integrated analysis of phosphoproteomic data and prediction of kinase activity revealed PAK4-PKC/SRC network to be highly deregulated in SCC but not in papilloma. This detailed molecular picture, both at the proteome and phosphoproteome level, will prove useful for the study of mechanisms of tumor progression.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Machesky, Professor Laura and Zanivan, Professor Sara Rossana and Tang, Mr Hao and Neilson, Ms Lisa and Kalna, Dr Gabriela
Authors: Zanivan, S., Meves, A., Behrendt, K., Schoof, E.M., Neilson, L.J., Cox, J., Tang, H.R., Kalna, G., van Ree, J.H., van Deursen, J.M., Trempus, C.S., Machesky, L.M., Linding, R., Wickström, S.A., Fässler, R., and Mann, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Cell Reports
ISSN:2211-1247
ISSN (Online):2211-1247

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