Vitronectin alters fibronectin organization at the cell–material interface

Gonzalez Garcia, C., Cantini, M. , Moratal, D., Altankov, G. and Salmerón-Sánchez, M. (2013) Vitronectin alters fibronectin organization at the cell–material interface. Colloids and Surfaces B: Biointerfaces, 111(1), pp. 618-625. (doi: 10.1016/j.colsurfb.2013.07.016)

Abstract

Cells assemble fibronectin (FN) into fibrils in a process mediated by integrins. For this process to occur, it is known that the presence of other serum proteins is necessary. However, the individual effect of these proteins on FN fibrillogenesis has not been addressed so far. In this study, the effect of vitronectin (VN), an ECM adhesion protein, on material-driven FN fibrillogenesis and cell-mediated FN reorganization is investigated. Poly(ethyl acrylate), PEA, which has previously shown the ability to induce the organization of FN into well-developed physiological-like networks upon adsorption, was employed as a material substrate. FN adsorption, cell adhesion and cellular FN reorganization in the presence or absence of VN were studied. Both FN surface density, quantified via western blot, and its distribution on PEA surfaces, determined via atomic force microscopy, were altered when FN was adsorbed competitively with VN at certain compositions. Moreover, the presence of VN on the material surfaces enhanced cell-mediated FN reorganization and secretion, in comparison with the process which took place in the presence of serum proteins.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Gonzalez Garcia, Dr Cristina and Cantini, Dr Marco
• Authors: Gonzalez Garcia, C., Cantini, M., Moratal, D., Altankov, G., and Salmerón-Sánchez, M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Colloids and Surfaces B: Biointerfaces
• ISSN: 0927-7765
• ISSN (Online): 1873-4367