A multi-object spectral imaging instrument

Gibson, G.M., Dienerowitz, M., Kelleher, P.A., Harvey, A.R. and Padgett, M.J. (2013) A multi-object spectral imaging instrument. Journal of Optics, 15(8), 085302. (doi:10.1088/2040-8978/15/8/085302)

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Abstract

We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dienerowitz, Dr Maria and Gibson, Dr Graham and Harvey, Professor Andrew and Padgett, Professor Miles
Authors: Gibson, G.M., Dienerowitz, M., Kelleher, P.A., Harvey, A.R., and Padgett, M.J.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Journal of Optics
Publisher:IOP Publishing Ltd.
ISSN:2040-8978
ISSN (Online):2040-8986
Copyright Holders:Copyright © 2013 IOP Publishing Ltd.
First Published:First published in the Journal of Optics 15(8):085302
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
Data DOI:10.5525/gla.researchdata.158

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
482571Multi-object, high-throughput, spectro-microscopy: Life Sciences InterfaceMiles PadgettEngineering & Physical Sciences Research Council (EPSRC)EP/H007636/1P&A - PHYSICS & ASTRONOMY