Influence of periodontal disease, porphyromonas gingivalisand cigarette smoking on systemic anti-citrullinated peptide antibody titres

Lappin, D.F., Apatzidou, D., Quirke, A.M., Oliver-Bell, J., Butcher, J.P., Kinane, D.F., Riggio, M.P. , Venables, P., McInnes, I.B. and Culshaw, S. (2013) Influence of periodontal disease, porphyromonas gingivalisand cigarette smoking on systemic anti-citrullinated peptide antibody titres. Journal of Clinical Periodontology, 40(10), pp. 907-915. (doi: 10.1111/jcpe.12138) (PMID:23902301)

Abstract

<p>Background: Anti-citrullinated protein antibody (ACPA) responses may precede clinical onset of rheumatoid arthritis. Porphyromonas gingivalispeptidylarginine deiminase can citrullinate proteins possibly inducing autoimmunity in susceptible individuals.</p> <p>Aim: To determine whether periodontitis, carriage of P. gingivalis, smoking and periodontal therapy influence ACPA titres.</p> <p>Methods: Serum and plaque samples were collected from 39 periodontitis patients before and after non-surgical periodontal treatment, and from 36 healthy subjects. Carriage of P. gingivalis was determined by PCR of plaque DNA. ACPA was determined by anti-cyclic citrullinated peptide (CCP) enzyme-linked immunosorbent assay (ELISA). Anti-P. gingivalis titres were determined by ELISA.</p> <p>Results: Untreated periodontitis patients had higher anti-CCP antibody titres than healthy controls [three patients (8%) greater than manufacturer suggested assay diagnostic threshold (5 Assay Units/AU) versus none (0%); mean ± SEM: 1.37 ± 0.23 versus 0.40 ± 0.10 AU, p < 0.0001]. Periodontitis patients who smoked demonstrated lower anti-P. gingivalis (15956 ± 4385 versus 2512 ± 1290 Units/ml, p < 0.05), but similar anti-CCP than non-smoking periodontitis patients (smokers: 1.31 ± 0.35; non-smokers: 1.41 ± 0.32 AU). Healthy smokers demonstrated elevated anti-CCP titres (0.75 ± 0.19 AU), at levels between healthy non-smokers (0.15 ± 0.05 AU) and non-smoker periodontitis patients. Six months after periodontal treatment, there were significant reductions in anti-CCP (non-smokers p < 0.05) and anti-P. gingivalis (all participants p < 0.01).</p> <p>Conclusion: In subjects with periodontitis, P. gingivalis infection may be responsible for inducing autoimmune responses that characterize rheumatoid arthritis.</p>

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInnes, Professor Iain and Lappin, Dr David and Oliver-Bell, Dr Jessica and Butcher, Mr John and Riggio, Professor Marcello and Culshaw, Professor Shauna
• Authors: Lappin, D.F., Apatzidou, D., Quirke, A.M., Oliver-Bell, J., Butcher, J.P., Kinane, D.F., Riggio, M.P., Venables, P., McInnes, I.B., and Culshaw, S.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: Journal of Clinical Periodontology
• ISSN: 0303-6979
• ISSN (Online): 1600-051X