Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline

Gabrielsen, M., Riboldi-Tunnicliffe, A., Ibáñez-Shimabukuro, M., Griffiths, K., Roe, A.J., Cooper, A., Smith, B., Córsico, B. and Kennedy, M.W. (2012) Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 68, pp. 939-941. (doi:10.1107/S1744309112026553)

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Abstract

As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of `microdomains'. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Malcolm and Riboldi-Tunnicliffe, Dr Alan and Gabrielsen, Dr Mads and Roe, Dr Andrew and Smith, Dr Brian and Cooper, Professor Alan
Authors: Gabrielsen, M., Riboldi-Tunnicliffe, A., Ibáñez-Shimabukuro, M., Griffiths, K., Roe, A.J., Cooper, A., Smith, B., Córsico, B., and Kennedy, M.W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Chemistry
College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
Publisher:International Union of Crystallography
ISSN:1744-3091
ISSN (Online):1744-3091
Copyright Holders:Copyright © 2012 International Union of Crystallography
First Published:First published in Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 68:939-941
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
465741Structural and biophysical analysis of novel lipid binding proteins from parasitic helminthsMalcolm KennedyWellcome Trust (WELLCOME)083625/Z/07/ZLS - ANIMAL BIOLOGY
491441A biochemical and molecular analysis of the YhaO membrane protein in Escherichia coli O157:H7Andrew RoeBiotechnology and Biological Sciences Research Council (BBSRC)BB/G011389/1III - BACTERIOLOGY