Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells

Kingham, E., White, K., Gadegaard, N. , Dalby, M.J. and Oreffo, R.O.C. (2013) Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells. Small, 9(12), pp. 2140-2151. (doi:10.1002/smll.201202340)

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Abstract

The production of bone-forming osteogenic cells for research purposes or transplantation therapies remains a significant challenge. Using planar polycarbonate substrates lacking in topographical cues and substrates displaying a nanotopographical pattern, mesenchymal differentiation of human embryonic stem cells is directed in the absence of chemical factors and without induction of differentiation by embryoid body formation. Cells incubated on nanotopographical substrates show enhanced expression of mesenchymal or stromal markers and expression of early osteogenic progenitors at levels above those detected in cells on planar substrates in the same basal media. Evidence of epithelial-to-mesenchymal transition during substrate differentiation and DNA methylation changes akin to chemical induction are also observed. These studies provide a suitable approach to overcome regenerative medical challenges and describe a defined, reproducible platform for human embryonic stem cell differentiation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and Gadegaard, Professor Nikolaj
Authors: Kingham, E., White, K., Gadegaard, N., Dalby, M.J., and Oreffo, R.O.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Small
Publisher:Elsevier
ISSN:1613-6810
ISSN (Online):1613-6829

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