Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications

Stalmach, A. , Albalat, A., Mullen, W. and Mischak, H. (2013) Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications. Electrophoresis, 34(11), pp. 1452-1464. (doi: 10.1002/elps.201200708)

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Publisher's URL: http://dx.doi.org/10.1002/elps.201200708

Abstract

Proteome analysis using capillary electrophoresis coupled to mass spectrometry (CE-MS) has been used in a number of clinical applications in the past years. The main focus of CE-MS-based studies has been on the investigation of urine, due to the stability of the urinary proteome, ease of collection, and also the low molecular weight range of the urinary proteome, mostly peptides below 30 kDa. The reproducibility of this approach has enabled analysis of over 20 000 samples in a comparable way, giving enormous statistical power to any additional study involving this methodological setup. In this article, we review the major technological issues associated with the application of CE-MS in the routine investigation of the urinary proteome for clinical applications. We pinpoint recent developments that may have a chance to improve on the currently used approach, and highlight obstacles that need to be solved. In the second part of the article, we review the recent clinical applications, aiming to highlight relevant issues, and possible future routine applications in clinical diagnosis. In the end, we provide a short outlook, and indicate future developments to be expected, as well as problems that need to be solved to enable routine application of CE-MS in a clinical setting.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stalmach, Dr Angelique and Albalat, Dr Amaya and Mullen, Dr Bill and Mischak, Professor Harald
Authors: Stalmach, A., Albalat, A., Mullen, W., and Mischak, H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Electrophoresis
ISSN:0173-0835
ISSN (Online):1522-2683
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
546181Development of an efficient, robust MS-based platform for early detection of acute kidney injuryHarald MischakMedical Research Council (MRC)G1000791RI CARDIOVASCULAR & MEDICAL SCIENCES