Effects of hydroxyapatite and PDGF concentrations on osteoblast growth in a nanohydroxyapatite-polylactic acid composite for guided tissue regeneration

Talal, A., McKay, I.J., Tanner, K.E. and Hughes, F.J. (2013) Effects of hydroxyapatite and PDGF concentrations on osteoblast growth in a nanohydroxyapatite-polylactic acid composite for guided tissue regeneration. Journal of Materials Science: Materials in Medicine, 24(9), pp. 2211-2221. (doi: 10.1007/s10856-013-4963-9)

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Abstract

The technique of guided tissue regeneration (GTR) has evolved over recent years in an attempt to achieve periodontal tissue regeneration by the use of a barrier membrane. However, there are significant limitations in the currently available membranes and overall outcomes may be limited. A degradable composite material was investigated as a potential GTR membrane material. Polylactic acid (PLA) and nanohydroxyapatite (nHA) composite was analysed, its bioactive potential and suitability as a carrier system for growth factors were assessed. The effect of nHA concentrations and the addition of platelet derived growth factor (PDGF) on osteoblast proliferation and differentiation was investigated. The bioactivity was dependent on the nHA concentration in the films, with more apatite deposited on films containing higher nHA content. Osteoblasts proliferated well on samples containing low nHA content and differentiated on films with higher nHA content. The composite films were able to deliver PDGF and cell proliferation increased on samples that were pre absorbed with the growth factor. nHA–PLA composite films are able to deliver active PDGF. In addition the bioactivity and cell differentiation was higher on films containing more nHA. The use of a nHA–PLA composite material containing a high concentration of nHA may be a useful material for GTR membrane as it will not only act as a barrier, but may also be able to enhance bone regeneration by delivery of biologically active molecules.

Item Type:Articles
Keywords:Guided tissue regeneration, polylactic acid, nano-hydroxyapatite, platelet derived growth factor, osteoblasts
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tanner, Professor Kathleen
Authors: Talal, A., McKay, I.J., Tanner, K.E., and Hughes, F.J.
Subjects:R Medicine > RK Dentistry
T Technology > T Technology (General)
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Research Group:Biomaterials and Tissue Engineering
Journal Name:Journal of Materials Science: Materials in Medicine
Publisher:Springer
ISSN:0957-4530
ISSN (Online):1573-4838
Copyright Holders:Copyright © 2013 Springer
First Published:First published in Journal of Materials Science: Materials in Medicine 2013
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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