Gantke, T., Boussouf, S., Janzen, J., Morrice, N.A., Howell, S., Muhlberger, E. and Ley, S.C. (2013) Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells. Biochemical Journal, 452(2), pp. 359-365. (doi: 10.1042/BJ20121873)
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Abstract
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)–ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]–NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Morrice, Dr Nicholas |
Authors: | Gantke, T., Boussouf, S., Janzen, J., Morrice, N.A., Howell, S., Muhlberger, E., and Ley, S.C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Biochemical Journal |
Publisher: | Portland Press Ltd. |
ISSN: | 0264-6021 |
Copyright Holders: | Copyright © 2013 The Authors |
First Published: | First published in Biochemical Journal 452(2):359-365 |
Publisher Policy: | Reproduced under a Creative Commons License |
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