Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide

de Bont, H.B., Liskamp, R.M. , O'Brian, C.A., Erkelens, C., Veeneman, G.H. and van Boom, J.H. (1989) Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide. International Journal of Peptide and Protein Research, 33(2), pp. 115-123. (doi:10.1111/j.1399-3011.1989.tb00196.x)

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Abstract

The syntheses of a protein kinase C (PKC) peptide substrate, H-Lys-Arg-Thr-Leu-Arg-OH, and a phosphopeptide analog of the synthetic substrate, H-Lys-Arg-Thr(P)-Leu-Arg-OH, are reported. PKC phosphorylates the peptide with an apparent KM of 0.30 +/- 0.04 mM and an apparent Vmax equal to one-tenth that of histone III-S. The synthesis of the phosphopeptide features a recently developed convenient phosphorylation procedure for serine and threonine using N,N-diethylamino-dibenzylphosphoramidite. A complete characterization of the PKC substrate and its corresponding phosphopeptide by C-H COSY 2D n.m.r. is included.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liskamp, Professor Robert
Authors: de Bont, H.B., Liskamp, R.M., O'Brian, C.A., Erkelens, C., Veeneman, G.H., and van Boom, J.H.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:International Journal of Peptide and Protein Research
ISSN:0367-8377
ISSN (Online):1747-0285

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