Antigen specific suppression of humoral immunity by anergic Ars/A1 B cells

Aviszus, K., Macleod, M. , Kirchenbaum, G.A., Detanico, D.O., Heiser, R.A., St. Clair, J.B., Guo, W.J. and Wysocki, L.J. (2012) Antigen specific suppression of humoral immunity by anergic Ars/A1 B cells. Journal of Immunology, 189(9), pp. 4275-4283.

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Abstract

Autoreactive anergic B lymphocytes are considered to be dangerous because of their potential for activation and recruitment into autoimmune responses. Yet they persist for days and constitute ~5% of the B cell pool. We assessed their functional potential in the Ars/A1 transgene model, where anergic B cells express a dual-reactive antigen receptor that binds, in addition to a self-antigen, the hapten p-azophenylarsonate (Ars). When Ars/A1 B cells were transferred into adoptive recipients that were immunized with foreign proteins covalently conjugated with Ars, endogenous IgG immune responses to both were selectively and severely diminished, and the development of T helper cells was impaired. Approximately 95% inhibition of the anti-Ars response was attained with ~4000 transferred Ars/A1 B cells through redundant mechanisms, one of which depended upon their expression of MHC II but not upon secretion of IL-10 or IgM. This antigen-specific suppressive activity implicates the autoreactive anergic B cell as an enforcer of immunological tolerance to self-antigens.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Macleod, Dr Megan
Authors: Aviszus, K., Macleod, M., Kirchenbaum, G.A., Detanico, D.O., Heiser, R.A., St. Clair, J.B., Guo, W.J., and Wysocki, L.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Immunology
ISSN:0022-1767
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