Sommerville, K. and Preston, T. (2001) Characterisation of dissolved combined amino acids in marine waters. Rapid Communications in Mass Spectrometry, 15(15), pp. 1287-1290. (doi: 10.1002/rcm.302)
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Publisher's URL: http://dx.doi.org/10.1002/rcm.302
Abstract
Dissolved combined amino acids (DCAA) are important constituents of the dissolved organic nitrogen (DON) pool in marine environments, although little is known about their sources, dynamics and sinks. The DCAA pool consists of various compounds including proteins and peptides, proteins linked to sugars and amino acids adsorbed to humic and fulvic acids, clays and other materials. The proportions of each of these components and the extent to which they are used by microplankton living within the photic zone are not known. An investigation was carried out, using N-15 isotope dilution techniques, to determine the concentration and composition of dissolved amino acid pools in the marine environment. A near- shore seawater sample was collected and split into fractions to determine the concentrations of dissolved free amino acids (DFAA), DCAA and a lt 3 kDa dissolved peptide fraction (DPEP; obtained by ultrafiltration). DCAA and DPEP fractions were hydrolysed to yield free amino acids and all samples were analysed by gas chromatography/mass spectrometry (GC/MS) as isobutyloxycarbonyl/tert-butyldimethylsilyl derivatives. The DFAA was the smallest fraction representing similar to1% of total dissolved amino acids. The majority of DCAA was contained in the low molecular weight DPEP fraction (90%) and was probably as a result of release from phytoplankton and degradation by heterotrophic bacteria.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Preston, Professor Tom |
Authors: | Sommerville, K., and Preston, T. |
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QD Chemistry |
College/School: | College of Science and Engineering > Scottish Universities Environmental Research Centre |
Journal Name: | Rapid Communications in Mass Spectrometry |
ISSN: | 0951-4198 |
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