Saliva and serum levels of B-cell activating factors and tumor necrosis factor-alpha in periodontitis patients

Gümüş, P., Nizam, N., Lappin, D.F. and Buduneli, N. (2014) Saliva and serum levels of B-cell activating factors and tumor necrosis factor-alpha in periodontitis patients. Journal of Periodontology, 85(2), pp. 270-280. (doi: 10.1902/jop.2013.130117)

Abstract

Aim: This study was conducted to evaluate saliva, serum levels of A proliferation-inducing ligand (APRIL), B-cell activating factor (BAFF), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-10 in patients with chronic periodontitis (CP) or aggressive periodontitis (AgP) and periodontally healthy (H) individuals.<p></p> Materials and Methods: Twenty-five patients with AgP, 20 patients with CP, 20 periodontally healthy individuals were included in the study. Smoking status was recorded and all individuals were divided into non-smoker and smoker groups. Saliva, serum samples were collected before clinical periodontal measurements. APRIL, BAFF, TNF-α, IL-6, IL-10 levels in serum, saliva samples were determined by ELISA. Statistical analysis was performed using multivariate analysis ANOVA and bivariate correlation.<p></p> Results: Serum, saliva levels of TNF-α, APRIL, BAFF, IL-6 and IL-10 were similar in CP and AgP groups. Serum levels of TNF-α, APRIL, BAFF and saliva levels of BAFF were significantly higher in periodontitis groups than healthy controls (p<0.05). Non-smoker CP, AgP patients had lower levels of saliva TNF-α, APRIL serum APRIL, IL-6 than smoker counterparts (p<0.05). Saliva APRIL, serum TNF-α, IL-6 levels were significantly higher in healthy smokers than healthy non-smokers (p<0.05). Clinical periodontal parameters correlated positively with TNF-family cytokines, and negatively with IL-10 (p<0.05).<p></p> Conclusion: Within the limits of this study, it may be suggested that elevated salivary, serum TNF-α, APRIL, BAFF, in periodontitis patients may contribute to the dominance of B cells in periodontitis lesions. Moreover, their higher levels in healthy smokers than non-smoker counterparts may play a role in detrimental effects of smoking on periodontal tissues.<p></p>

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Lappin, Dr David
• Authors: Gümüş, P., Nizam, N., Lappin, D.F., and Buduneli, N.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Journal of Periodontology
• Publisher: American Academy of Periodontology
• ISSN: 0022-3492
• ISSN (Online): 1943-3670