Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P. and Magennis, S.W. (2011) Global structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133(5), pp. 1188-1191. (doi: 10.1021/ja108626w)
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Abstract
Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA−protein interaction studies.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Magennis, Dr Steven |
| Authors: | Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P., and Magennis, S.W. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Journal of the American Chemical Society |
| Publisher: | American Chemical Society |
| ISSN: | 0002-7863 |
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