Modularity of plant metabolic gene clusters: a trio of linked genes that are collectively required for acylation of triterpenes in oat

Mugford, S.T. et al. (2013) Modularity of plant metabolic gene clusters: a trio of linked genes that are collectively required for acylation of triterpenes in oat. Plant Cell, 25(3), pp. 1078-1092. (doi:10.1105/tpc.113.110551)

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Abstract

Operon-like gene clusters are an emerging phenomenon in the field of plant natural products. The genes encoding some of the best-characterized plant secondary metabolite biosynthetic pathways are scattered across plant genomes. However, an increasing number of gene clusters encoding the synthesis of diverse natural products have recently been reported in plant genomes. These clusters have arisen through the neo-functionalization and relocation of existing genes within the genome, and not by horizontal gene transfer from microbes. The reasons for clustering are not yet clear, although this form of gene organization is likely to facilitate co-inheritance and co-regulation. Oats (Avena spp) synthesize antimicrobial triterpenoids (avenacins) that provide protection against disease. The synthesis of these compounds is encoded by a gene cluster. Here we show that a module of three adjacent genes within the wider biosynthetic gene cluster is required for avenacin acylation. Through the characterization of these genes and their encoded proteins we present a model of the subcellular organization of triterpenoid biosynthesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rosser, Professor Susan
Authors: Mugford, S.T., Louveau, T., Melton, R., Qi, X., Bakht, S., Hill, L., Tsurushima, T., Honkanen, S., Rosser, S.J., Lomonossoff, G.P., and Osbourn, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Cell
Publisher:American Society of Plant Biologists
ISSN:1040-4651
ISSN (Online):1532-298X
Copyright Holders:Copyright © 2013 American Society of Plant Biologists
First Published:First published in Plant Cell 25(3):1078-1092
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
519111Sandpit: Synthetic integrons for continuous directed evolution of complex genetic ensemblesSusan RosserEngineering & Physical Sciences Research Council (EPSRC)EP/H019154/1RI MOLECULAR CELL & SYSTEMS BIOLOGY