TLR-mediated stimulation of APC: distinct cytokine responses of B cells and dendritic cells

Barr, T.A., Brown, S., Ryan, G., Zhao, J. and Gray, D. (2007) TLR-mediated stimulation of APC: distinct cytokine responses of B cells and dendritic cells. European Journal of Immunology, 37(11), pp. 3040-3053. (doi: 10.1002/eji.200636483)

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In addition to their role in humoral immunity, B lymphocytes are important antigen-presenting cells (APC). In the same way as other APC, B cells make cytokines upon activation and have the potential to modulate T cell responses. In this study, we investigated which mouse B cell subsets are the most potent cytokine producers, and examined the role of Toll-like receptors (TLR) in the control of secretion of IL-6, IL-10, IL-12 and IFN-γ by B cells. Production of some cytokines was restricted to particular subsets. Marginal zone and B1 cells were the predominant source of B cell IL-10 in the spleen. Conversely, follicular B cells were found to express IFN-γ mRNA directly ex vivo. The nature of the activating stimulus dramatically influenced the cytokine made by B cells. Thus, in response to combined TLR stimulation, or via phorbol esters, IFN-γ was secreted. IL-10 was elicited by T-dependent activation or stimulation through TLR2, 4 or 9. This pattern of cytokine expression contrasts with that elicited from dendritic cells. QRT-PCR array data indicate that this may be due to differential expression of TLR signalling molecules, effectors and adaptors. Our data highlight the potentially unique nature of immune modulation when B cells act as APC.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Barr, Dr Tom
Authors: Barr, T.A., Brown, S., Ryan, G., Zhao, J., and Gray, D.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:European Journal of Immunology

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