Guabiraba, R., Russo, R.C., Coelho, A.M., Ferreira, M.A.N.D., Lopes, G.A.O., Gomes, A.K.C., Andrade, S.P., Barcelos, L.S. and Teixeira, M.M. (2013) Blockade of cannabinoid receptors reduces inflammation, leukocyte accumulation and neovascularization in a model of sponge-induced inflammatory angiogenesis. Inflammation Research, 62(8), pp. 811-821. (doi: 10.1007/s00011-013-0638-8)
Full text not currently available from Enlighten.
Publisher's URL: http://dx.doi.org/10.1007/s00011-013-0638-8
Abstract
<p>Objective: Angiogenesis depends on a complex interaction between cellular networks and mediators. The endocannabinoid system and its receptors have been shown to play a role in models of inflammation. Here, we investigated whether blockade of cannabinoid receptors may interfere with inflammatory angiogenesis.</p> <p>Materials and methods: Polyester-polyurethane sponges were implanted in C57Bl/6j mice. Animals received doses (3 and 10 mg/kg/daily, s.c.) of the cannabinoid receptor antagonists SR141716A (CB1) or SR144528 (CB2). Implants were collected at days 7 and 14 for cytokines, hemoglobin, myeloperoxidase, and N-acetylglucosaminidase measurements, as indices of inflammation, angiogenesis, neutrophil and macrophage accumulation, respectively. Histological and morphometric analysis were also performed.</p> <p>Results: Cannabinoid receptors expression in implants was detected from day 4 after implantation. Treatment with CB1 or CB2 receptor antagonists reduced cellular influx into sponges at days 7 and 14 after implantation, although CB1 receptor antagonist were more effective at blocking leukocyte accumulation. There was a reduction in TNF-α, VEGF, CXCL1/KC, CCL2/JE, and CCL3/MIP-1α levels, with increase in CCL5/RANTES. Both treatments reduced neovascularization. Dual blockade of cannabinoid receptors resulted in maximum inhibition of inflammatory angiogenesis.</p> <p>Conclusions: Blockade of cannabinoid receptors reduced leukocyte accumulation, inflammation and neovascularization, suggesting an important role of endocannabinoids in sponge-induced inflammatory angiogenesis both via CB1 and CB2 receptors.</p>
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Guabiraba Brito, Dr Rodrigo |
Authors: | Guabiraba, R., Russo, R.C., Coelho, A.M., Ferreira, M.A.N.D., Lopes, G.A.O., Gomes, A.K.C., Andrade, S.P., Barcelos, L.S., and Teixeira, M.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Inflammation Research |
ISSN: | 1023-3830 |
ISSN (Online): | 1420-908X |
University Staff: Request a correction | Enlighten Editors: Update this record