Rheology at the micro-scale: new tools for bio-analysis

Warren, R.L., Tassieri, M. , Li, X., Glidle, A., Paterson, D.J., Carlsson, A. and Cooper, J.M. (2013) Rheology at the micro-scale: new tools for bio-analysis. In: Optical Methods for Inspection, Characterization and Imaging of Biomaterials, Munich, Germany, 13-16 May 2013, (doi:10.1117/12.2025254)

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Publisher's URL: http://dx.doi.org/10.1117/12.2025254

Abstract

We present a simple and non-invasive experimental procedure to measure the linear viscoelastic properties of cells by passive particle tracking microrheology. In order to do this, a generalised Langevin equation is adopted to relate the timedependent thermal fluctuations of a probe sensor, immobilised to the cell’s membrane, to the frequency-dependent viscoelastic moduli of the cell. The method has been validated by measuring the linear viscoelastic response of a soft solid and then applied to cell physiology studies. It is shown that the viscoelastic moduli are related to the cell’s cytoskeletal structure, which in this work is modulated either by inhibiting the actin/myosin-II interactions by means of blebbistatin or by varying the solution osmolarity from iso- to hypo-osmotic conditions. The insights gained from this form of rheological analysis promises to be a valuable addition to physiological studies; e.g. cell physiology during pathology and pharmacological response.

Item Type:Conference Proceedings
Additional Information:Copyright 2013 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2025254
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Warren, Ms Rebecca and Tassieri, Dr Manlio and Glidle, Dr Andrew and Carlsson, Dr Allan and Cooper, Professor Jonathan
Authors: Warren, R.L., Tassieri, M., Li, X., Glidle, A., Paterson, D.J., Carlsson, A., and Cooper, J.M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
ISSN:0277786X
Copyright Holders:Copyright © 2013 SPIE
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
465001Listening to the Microworld.Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F040857/1ENG - BIOMEDICAL ENGINEERING
387191Radical Solutions for Researching the Proteome (RASOR)Andrew PittBiotechnology and Biological Sciences Research Council (BBSRC)BB/C511572/1RI MOLECULAR CELL & SYSTEMS BIOLOGY