ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions

Roussakis, E., Voutsadaki, S., Pinakoulaki, E., Sideris, D.P., Tokatlidis, K. and Katerinopoulos, H.E. (2008) ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions. Cell Calcium, 44(3), pp. 270-275. (doi:10.1016/j.ceca.2007.12.008)

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Abstract

A new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3′,2′:3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tokatlidis, Professor Kostas
Authors: Roussakis, E., Voutsadaki, S., Pinakoulaki, E., Sideris, D.P., Tokatlidis, K., and Katerinopoulos, H.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Cell Calcium
ISSN:0143-4160

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