Billecocq, A., Gauliard, N., Le May, N., Elliott, R.M., Flick, R. and Bouloy, M. (2008) RNA polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses. Virology, 378(2), pp. 377-384. (doi: 10.1016/j.virol.2008.05.033)
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Abstract
Rift Valley fever virus (RVFV, Bunyaviridae, Phlebovirus) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into the Arabian Peninsula in 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based on the use of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10<sup>7</sup> to 10<sup>8</sup> pfu/ml. We used the RNA polymerase I-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Elliott, Professor Richard |
Authors: | Billecocq, A., Gauliard, N., Le May, N., Elliott, R.M., Flick, R., and Bouloy, M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Virology |
ISSN: | 0042-6822 |
ISSN (Online): | 1096-0341 |
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