Shi, X. , Goli, J., Clark, G., Brauburger, K. and Elliott, R.M. (2009) Functional analysis of the Bunyamwera orthobunyavirus Gc glycoprotein. Journal of General Virology, 90(10), pp. 2483-2492. (doi: 10.1099/vir.0.013540-0)
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Abstract
The virion glycoproteins Gn and Gc of Bunyamwera orthobunyavirus (family Bunyaviridae) are encoded by the M RNA genome segment and have roles in both viral attachment and membrane fusion. To investigate further the structure and function of the Gc protein in viral replication, we generated 12 mutants that contain truncations from the N terminus. The effects of these deletions were analysed with regard to Golgi targeting, low pH-dependent membrane fusion, infectious virus-like particle (VLP) formation and virus infectivity. Our results show that the N-terminal half (453 residues) of the Gc ectodomain (909 residues in total) is dispensable for Golgi trafficking and cell fusion. However, deletions in this region resulted in a significant reduction in VLP formation. Four mutant viruses that contained N-terminal deletions in their Gc proteins were rescued, and found to be attenuated to different degrees in BHK-21 cells. Taken together, our data indicate that the N-terminal half of the Gc ectodomain is dispensable for replication in cell culture, whereas the C-terminal half is required to mediate cell fusion. A model for the domain structure of the Gc ectodomain is proposed.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Elliott, Professor Richard and Shi, Dr Xiaohong |
Authors: | Shi, X., Goli, J., Clark, G., Brauburger, K., and Elliott, R.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Journal of General Virology |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1465-2099 |
Copyright Holders: | Copyright © 2009 SGM |
First Published: | First published in Journal of General Virology 90(10):2483-2492 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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