Bowden, T.A., Bitto, D., McLees, A., Yeromonahos, C., Elliott, R.M. and Huiskonen, J.T. (2013) Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike. PLoS Pathogens, 9(5), e1003374. (doi: 10.1371/journal.ppat.1003374)
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Abstract
The genus <i>Orthobunyavirus</i> within the family <i>Bunyaviridae</i> constitutes an expanding group of emerging viruses, which threaten human and animal health. Despite the medical importance, little is known about orthobunyavirus structure, a prerequisite for understanding virus assembly and entry. Here, using electron cryo-tomography, we report the ultrastructure of Bunyamwera virus, the prototypic member of this genus. Whilst Bunyamwera virions are pleomorphic in shape, they display a locally ordered lattice of glycoprotein spikes. Each spike protrudes 18 nm from the viral membrane and becomes disordered upon introduction to an acidic environment. Using sub-tomogram averaging, we derived a three-dimensional model of the trimeric pre-fusion glycoprotein spike to 3-nm resolution. The glycoprotein spike consists mainly of the putative class-II fusion glycoprotein and exhibits a unique tripod-like arrangement. Protein–protein contacts between neighbouring spikes occur at membrane-proximal regions and intra-spike contacts at membrane-distal regions. This trimeric assembly deviates from previously observed fusion glycoprotein arrangements, suggesting a greater than anticipated repertoire of viral fusion glycoprotein oligomerization. Our study provides evidence of a pH-dependent conformational change that occurs during orthobunyaviral entry into host cells and a blueprint for the structure of this group of emerging pathogens.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Elliott, Professor Richard |
Authors: | Bowden, T.A., Bitto, D., McLees, A., Yeromonahos, C., Elliott, R.M., and Huiskonen, J.T. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | PLoS Pathogens |
Publisher: | Public Library of Science |
ISSN: | 1553-7366 |
ISSN (Online): | 1553-7374 |
Copyright Holders: | Copyright © 2013 The Authors |
First Published: | First published in PLoS ONE 9(5):e1003374 |
Publisher Policy: | Reproduced under a Creative Commons License |
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