GPCR oligomerization and receptor trafficking

Ward, R.W., Xu, T.-X. and Milligan, G. (2013) GPCR oligomerization and receptor trafficking. Methods in Enzymology, 521, pp. 69-90. (doi: 10.1016/B978-0-12-391862-8.00004-1)

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Abstract

The effects of oligomerization of G protein-coupled receptors (GPCRs) upon their trafficking around the cell are considerable, and this raises the potential of significant impact upon the use of existing pharmacological agents and the development of new ones. Herein, we describe a number of different techniques that can be used to study receptor dimerization/oligomerization and trafficking, beginning with a cellular system which allows the expression of two GPCRs simultaneously, one under inducible control. Subsequently, we describe means to visualize and monitor the movement of GPCRs within the cell, detect oligomerization by both resonance energy transfer and more traditional biochemical approaches, and to measure the internalization of GPCRs as part of the process of receptor regulation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Xu, Professor Tianrui and Ward, Dr Richard and Milligan, Professor Graeme
Authors: Ward, R.W., Xu, T.-X., and Milligan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Methods in Enzymology
ISSN:0076-6879

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