Adhesion inhibition of F1C-fimbriated escherichia coli and pseudomonas aeruginosa PAK and PAO by multivalent carbohydrate ligands

Autar, R., Khan, A.S., Schad, M., Hacker, J., Liskamp, R.M.J. and Pieters, R.J. (2003) Adhesion inhibition of F1C-fimbriated escherichia coli and pseudomonas aeruginosa PAK and PAO by multivalent carbohydrate ligands. ChemBioChem, 4(12), pp. 1317-1325. (doi:10.1002/cbic.200300719)

Full text not currently available from Enlighten.

Abstract

In order to evaluate their inhibition of bacterial adhesion, the carbohydrate sequences GalNAcβ1→4Gal and GalNAcβ1→4Galβ1→4Glc were synthesized. The disaccharide was conjugated to dendrons based on the 3,5-di-(2-aminoethoxy)-benzoic acid branching unit to yield di- and tetravalent versions of these compounds. A divalent compound was also prepared that had significantly longer spacer arms. Relevant monovalent compounds were prepared for comparison. Their anti-adhesion properties against F1C-fimbriated uropathogenic Escherichia coli were evaluated in an ELISA-type assay by using a recombinant strain and also by using Pseudomonas aeruginosa strains PAO and PAK. Adhesion inhibition was observed in all cases, and multivalency effects of up to one order of magnitude were observed. The combination of spacer and multivalency effects led to a 38-fold increase in the potency of a divalent inhibitor with long spacer arms towards the PAO strain when compared with the free carbohydrate.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liskamp, Professor Robert
Authors: Autar, R., Khan, A.S., Schad, M., Hacker, J., Liskamp, R.M.J., and Pieters, R.J.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:ChemBioChem
ISSN:1439-4227
ISSN (Online):1439-7633

University Staff: Request a correction | Enlighten Editors: Update this record