Selective enrichment of ser-/thr-phosphorylated peptides in the presence of ser-/thr-glycosylated peptides

Poot, A.J., Ruijter, E., Nuijens, T., Dirksen, E.H.C., Heck, A.J.R., Slijper, M., Rijkers, D.T.S. and Liskamp, R.M.J. (2006) Selective enrichment of ser-/thr-phosphorylated peptides in the presence of ser-/thr-glycosylated peptides. Proteomics, 6(24), pp. 6394-6399. (doi:10.1002/pmic.200600373)

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Abstract

Modification through β-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to β-elimination as well. Therefore, we have optimized these conditions to achieve a β-elimination that is highly selective for phosphorylated peptides. This is the first report of selective β-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liskamp, Professor Robert
Authors: Poot, A.J., Ruijter, E., Nuijens, T., Dirksen, E.H.C., Heck, A.J.R., Slijper, M., Rijkers, D.T.S., and Liskamp, R.M.J.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Proteomics
ISSN:1615-9853
ISSN (Online):1615-9861

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