Magnetic resonance imaging of mass transport and structure inside a phototrophic biofilm

Ramanan, B., Holmes, W.M., Sloan, W.T. and Phoenix, V.R. (2013) Magnetic resonance imaging of mass transport and structure inside a phototrophic biofilm. Current Microbiology, 66(5), pp. 456-461. (doi:10.1007/s00284-012-0292-3)

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Abstract

The aim of this study was to utilize magnetic resonance imaging (MRI) to image structural heterogeneity and mass transport inside a biofilm which was too thick for photon based imaging. MRI was used to map water diffusion and image the transport of the paramagnetically tagged macromolecule, Gd-DTPA, inside a 2.5 mm thick cyanobacterial biofilm. The structural heterogeneity of the biofilm was imaged at resolutions down to 22 × 22 μm, enabling the impact of biofilm architecture on the mass transport of both water and Gd-DTPA to be investigated. Higher density areas of the biofilm correlated with areas exhibiting lower relative water diffusion coefficients and slower transport of Gd-DTPA, highlighting the impact of biofilm structure on mass transport phenomena. This approach has potential for shedding light on heterogeneous mass transport of a range of molecular mass molecules in biofilms.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Holmes, Dr William and Phoenix, Dr Vernon and Sloan, Mr William
Authors: Ramanan, B., Holmes, W.M., Sloan, W.T., and Phoenix, V.R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Public Health
College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:Current Microbiology
ISSN:0343-8651
ISSN (Online):1432-0991

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