Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces

Seras-Franzoso, J., Tsimbouri, P.M. , Burgess, K.V., Unzueta, U., Garcia-Fruitos, E., Vazquez, E., Villaverde, A. and Dalby, M.J. (2013) Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces. Nanomedicine, 9(2), pp. 207-220. (doi:10.2217/nnm.13.43) (PMID:23631503)

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Abstract

Aim: Bacterial inclusion bodies (IBs) are nanostructured (submicron), pseudospherical proteinaceous particles produced in recombinant bacteria resulting from ordered protein aggregation. Being mechanically stable, several physicochemical and biological properties of IBs can be tuned by appropriate selection of the producer strain and of culture conditions. It has been previously shown that IBs favor cell adhesion and surface colonization by mammalian cell lines upon decoration on materials surfaces, but how these biomaterials could influence the behavior of mesenchymal stem cells remains to be explored. Materials & methods: Here, the authors vary topography, stiffness and wettability using the IBs to decorate polycaprolactone surfaces on which mesenchymal stem cells are cultured. Results: The authors show that these topographies can be used to specifically target osteogenesis from mesenchymal stem cells, and through metabolomics, they show that the cells have increased energy demand during this bone-related differentiation. Conclusion: IBs as topographies can be used not only to direct cell proliferation but also to target differentiation of mesenchymal stem cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew and Burgess, Dr Karl
Authors: Seras-Franzoso, J., Tsimbouri, P.M., Burgess, K.V., Unzueta, U., Garcia-Fruitos, E., Vazquez, E., Villaverde, A., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nanomedicine
Publisher:Future Medicine Ltd.
ISSN:1743-5889
ISSN (Online):1748-6963

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