Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050

Böhm, P.S., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050. Journal of Physical Chemistry B, 117(11), pp. 3120-3126. (doi:10.1021/jp4005218)

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Abstract

We have revisited the RC-LH1 complex from Rhodopseudomonas (Rps.) acidophila for single-molecule spectroscopy. For the current study the pigment–protein complexes were stabilized in the detergent buffer solution using a relatively mild detergent (dodecyl-β-d-maltoside (DDM) instead of lauryldimethylamine N-oxide (LDAO)). This leads to a significant reduction of the fraction of broken/dissociated RC-LH1 complexes with respect to previous studies and has allowed us to investigate a sufficiently large sample of individual RC-LH1 complexes. For most of the complexes the fluorescence–excitation spectra exhibit a narrow spectral feature at the red end of the spectrum. Analysis of the statistics of the spectral properties yields a close resemblance with the results obtained on RC-LH1 complexes from Rps. palustris for which a low-resolution X-ray structure is available. Based on this comparison we come to the conclusion that for both species the RC-LH1 complex can be described by the same structural model, that is, an overall elliptical assembly of pigments that features a gap.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard and Southall, Mrs June
Authors: Böhm, P.S., Southall, J., Cogdell, R.J., and Köhler, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Physical Chemistry B
ISSN:1520-6106
ISSN (Online):1520-5207
Published Online:27 February 2013

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