Böhm, P.S., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050. Journal of Physical Chemistry B, 117(11), pp. 3120-3126. (doi: 10.1021/jp4005218)
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Abstract
We have revisited the RC-LH1 complex from Rhodopseudomonas (Rps.) acidophila for single-molecule spectroscopy. For the current study the pigment–protein complexes were stabilized in the detergent buffer solution using a relatively mild detergent (dodecyl-β-d-maltoside (DDM) instead of lauryldimethylamine N-oxide (LDAO)). This leads to a significant reduction of the fraction of broken/dissociated RC-LH1 complexes with respect to previous studies and has allowed us to investigate a sufficiently large sample of individual RC-LH1 complexes. For most of the complexes the fluorescence–excitation spectra exhibit a narrow spectral feature at the red end of the spectrum. Analysis of the statistics of the spectral properties yields a close resemblance with the results obtained on RC-LH1 complexes from Rps. palustris for which a low-resolution X-ray structure is available. Based on this comparison we come to the conclusion that for both species the RC-LH1 complex can be described by the same structural model, that is, an overall elliptical assembly of pigments that features a gap.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Cogdell, Professor Richard and Southall, Mrs June |
Authors: | Böhm, P.S., Southall, J., Cogdell, R.J., and Köhler, J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Journal of Physical Chemistry B |
ISSN: | 1520-6106 |
ISSN (Online): | 1520-5207 |
Published Online: | 27 February 2013 |
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